# Microbiome Gazers Episode 3
Author: BugSpeaks
Author URL: https://www.bugspeaks.com/blog/author/bugspeaks
Published: 2020-10-14
Category: General
Category URL: https://www.bugspeaks.com/blog/category/general
Meta Title: Microbiome Gazers Episode 3
URL: https://www.bugspeaks.com/blog/microbiome-gazers-episode-3

This is the third episode of the 'Microbiome Gazers'. Proudly hosted and organized by BugSpeaks® and Leucine Rich Bio.

Here Co-founders of Leucine Rich Bio Dr Debojyoti Dhar \[as guest\] and Mr Kumar Sankaran \[as host\] talk about the technologies – both sequencing and computational analysis – used within the field of microbiome.

**Highlights:**

- They begin with a brief comparison between the traditional microbiology / culture based approached and modern microbiome-based methodology.
- Factors involved in culturing-based methods and the associated drawbacks, especially regarding the inability to culture close to 2000 plus species that might thrive in a gut.
- Early technological walkthrough from Fluorescent In Situ Hybridization (FISH) to early PCR based amplicon sequencing, and other DNA based sequencing methods.
- About current technologies used – Amplicon \[mostly rRNA\] sequencing, whole genome metagenomics.
- Quick insights on meta-transcriptomics technology.
- Differences between using short read vs long read sequencing technologies.
- Details on various analysis platforms or suites available for analysing the metagenomic data, with a general follow through of major steps involved during a metagenomics analysis.
- Thoughts on the criticality of sample collection and extraction of DNA/RNA, and its impact on bioinformatic analysis.
- Thoughts on the criticality of reference databases.
- Thoughts on MetaRich - Leucine Rich Bio’s suite of metagenomic analysis suite, with specific focus on MetaRef \[Microorganism reference database\], MicroByte \[database of associations between diseases and microbiome\] and NutriBite \[ database of associations between nutrition and microbiome\].
- They wrap up by answering questions from attendees.


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